CCFS Media Prep Technician

Responsibilities:

• Work with Cell Culture and Fermentation Suite scientists to prepare cell culture media, feed, stocks, buffers and reagents
• Primary duties will include preparing many types of biological cell culture basal media, nutrient feed and stock solutions for NS0, CHO and hybridoma cells
• Solutions will be tested for osmolarity, pH, and turbidity
• Using aseptic technique and a biological safety cabinet, filter or pump solution into sterile containers as requested by scientists and store all solutions properly
• Lab instrument maintenance for Nova, Cedex, BGA, pH meter and osmometer as schedule permits
• Monitor stock solution inventory and prepare as needed
• Label all solutions according to CCFS guidelines
• Work with scientists to prepare custom solutions and make modifications as needed to standard media and feed formulations
• Update the Solution Preparation Console daily to reflect all lot numbers, catalog numbers and formulation modifications as well as storage conditions
• Calibrate laboratory equipment as required
• Clean and sterilize BSC according to schedule
• Monitor inventory in cold room and feed cabinet, discarding expired solutions and maintaining stock of general use solutions
• Maintain inventory of all chemicals in the cold room and media prep laboratory
• Receive chemicals, barcode, and log into the Chemical Inventory Console
• Retire chemicals from the inventory console as needed
• Provide support with the design of small bioreactor performance of experiments on media stability
• Assist in the preparation of laboratory SOPs
• Maintain work environment to guarantee the success of aseptic operations
• Record and keep accurate material inventory and update database
• Keep laboratory notebooks up to date
• Communicate effectively with internal customers
• Maintain training and compliance in areas of health and safety, security, environmental and operational aspects of daily activities in the working environment
• May require support of on-call service for specific research units in the lab areas. Hours are between 5:00pm-7:30am Monday thru Sunday. Tasks include but are not limited to transfer of contents from failing fridges and incubators to designated back up units, photograph original items, properly document failure, provide a work order of failing unit to facilities team and inform respective end-users about the transfer via email based on the SOP instructions

Nothing in this job description restricts management’s right to assign or reassign duties and responsibilities of this job at any time

Critical Skills:

• Demonstrated technical skills utilizing balances, pH meters, liquid-transfer devices
• Sufficient math skills to calculate Mass/Molarity, concentrations, and dilutions

• Attention to detail and understanding of the basic laboratory environment
• Excellent organizational skills; ability to troubleshoot and solve problems independently
• Ability to follow oral and written directions
• Proficient with MS Office: Excel, Word, Outlook, and PowerPoint

Basic Qualifications:

• Bachelor Degree in a chemistry, biology, or other relevant discipline with 0-1 year of experience in a laboratory environment OR
• Associate Degree in a chemistry, biology, or other relevant discipline with 2 years’ experience in a laboratory environment OR
• High School Degree or equivalent with 5 years of experience in a laboratory environment

Preferred Qualifications:

• Media Prep experience in a pharmaceutical laboratory environment
• Deep understanding of the basic chemistry of buffer preparation including titrations and reactions

Working Environment:

• Must be able to work in a laboratory, controlled environments requiring personal protective equipment (e.g., lab coat, safety glasses, etc.) in laboratory.
• Job pace may be fast and job completion demands may be high.
• Must be able to remain in a stationary position more than 25% of the time

• The person needs to occasionally move between labs, corridors, adjoining rooms, and buildings onsite.
• Frequently operate on instruments, objects, tools or controls, which will require regularly bending, squatting, stretching and reaching in order to perform in a service function.
• Occasionally move or lift up to 25 pounds (potential for occasional lifting of up to 50 pounds).
• Specific vision abilities required by this position include without limitation, the ability to observe details at close range distance vision, color vision, peripheral vision, depth perception, and ability to adjust focus in order to perform the essential service functions of this position.
• Occasionally operates a computer and other office machinery, such as a calculator, copy machine, and computer printer.
• Employee may be required to handle hazardous waste according to local, state, and federal regulations. Duties may include identifying, handling, generating, accumulating, storing and labeling hazardous waste.