Molecular Biologist

Summary:

We are seeking a highly motivated and organized Scientist II to contribute to the research and development of monoclonal antibodies in our Antibody Technologies team. This individual will be responsible for protein expression and purification; tissue culture and cell-line engineering; and testing antibodies for use in flow cytometry application. The position requires extensive hands-on experience with protein purification, tissue culture, protein expression, and a strong understanding of biochemistry and molecular biology.

Duties:

• Responsible for purification of antibodies and recombinant proteins using AKTA FPLC and manual methods. Must execute and document work using SOPs and must follow strict GMP requirements under ISO 13485 standards. Coordinates preparation and transfer of purified antibody and protein samples to other functional teams.
• Executes analytical testing of purified antibodies and proteins using SEC, SDS-PAGE, Western blot, MADLS, and qPCR.
• Responsible for transfection and/or expression of antibodies and recombinant proteins from various mammalian cell lines, including but not limited to CHO and hybridomas as well as E. coli. Specific requirement for hands-on experience with aseptic technique and cell-culture techniques
• Responsible for the execution of experiments with varying complexity using advanced molecular biology techniques.
• Responsible for the execution of experiments with varying complexity using multi-color Flow Cytometry.
• Analyze data using FCS Express and FlowJo software, report test results including completion of all required documentation and database entries as required by protocols and SOPs.

Qualification:

• A Bachelor’s or Master of Science degree in biochemistry, cellular or molecular biology, immunology, or related discipline and a minimum of 2-4 years of laboratory experience required. Protein purification, molecular biology and tissue culture experience required.
• Proficient in tissue culture techniques; including transfection of cells for protein expression.
• Proficient with aseptic technique and cell-culture techniques is required.
• Protein expression using various systems including but not limited to mammalian cells, insect cells, and bacteria.
• Protein purification with liquid chromatography and quality assurance using various analytical methods.
• Software: BD FACS Diva, FlowJo, FCS Express, and Microsoft Office Suite.

Preferred:

• Familiarity with assay automation.
• Experience with cell line engineering
• Hand-on experience with Flow Cytometry and the ability of analyzing Flow data is preferred.